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Dietary Fat and Phospholipase A₂ Activity of Sprague-Dawley Rat Large Intestine¹

Nutrition Program, State University of New York at Buffalo, Buffalo, NY 14214

The present studies were conducted to examine the effect of dietary lipid content and composition [(n-6) vs. (n-3) fatty acids] on the activity of mucosal phospholipase (PL)A₂ of the large intestinal tract of rats. Three segments of the large intestinal tract were examined: cecum, proximal colon and distal colon. Weanling male Sprague-Dawley rats were fed diets containing either 5% (LS) or 16% safflower (HS) or 14% menhaden oil plus 2% safflower oil (HM) for 3 wk with the oil replacing starch in the HS and HM diets on a weight basis. The lipid extracts of microsomal fractions from mucosal scrapings were examined for phospholipid and cholesterol content and fatty acid composition. Phospholipase A₂ was assayed using a fluorescent substrate. Rats fed the high fat diets had lower PLA₂ specific activities. The (n-3) or (n-6) fatty acid enrichment of the membranes had no effect of the activity of the enzyme. The activity of the enzyme decreased aborally from the cecum to the distal colon; the proximal colon had an intermediate specific activity.

KEY WORDS: • phospholipase A₂ • fatty acids • large intestine • membrane lipids • rats

¹ The research was partially supported by a grant from Buffalo General Hospital, Troup Fund #229809.

Manuscript received 23 April 1990. Revision accepted 30 October 1990.

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