QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Odle, J. Articles by Crenshaw, T. D. Search for Related Content PubMed PubMed Citation Articles by Odle, J. Articles by Crenshaw, T. D.

Postnatal Age and the Metabolism of Medium- and Long-Chain Fatty Acids by Isolated Hepatocytes from Small-for-Gestational-Age and Appropriate-for-Gestational-Age Piglets^1,2,

Jack Odle^*,,3, Norlin J. Benevenga^*, and Thomas D. Crenshaw

^* Department of Nutritional Science Department of Meat and Animal Science University of Wisconsin, Madison, WI 53706

Hepatocytes were isolated from full-term small-for-gestational-age (SGA) and appropriate-for-gestational-age (AGA) piglets at 6 or 48 h postpartum and incubated with 1 mmol/L [1-¹⁴C]-octanoate (8:0), -nonanoate (9:0) or -oleate (18:1). The cells oxidized [nmol 1-C/(h-10⁶ cells)] 9:0 to carbon dioxide (12.5) and acid soluble products (28.9) faster than 8:0 (10.9, 20.6, respectively), and both were oxidized faster than 18:1 (3.9, 9.9) regardless of the piglet age or weight. Oleate accumulated in lipid products eightfold faster than did 8:0 and 9:0. No differences between cells from SGA and AGA piglets were detected. Recovery of 1-C in CO₂ was 48% higher in incubations with cells from 48-h-old than from 6-h-old piglets. This increase was attributable to a 70% higher O₂ consumption by 48-h-old cells. Theoretical O₂ consumption rates were computed from the fatty acid flux data and compared with measured O₂ consumption. Hepatocytes from SGA and AGA piglets were equally capable of satisfying >75% of their energy needs from fatty acid oxidation. The O₂ consumption attributable to 9:0 metabolism was 30% higher than observed for 8:0 and 18:1. All fatty acids apparently spared endogenous fuels to a greater degree in 6-h-old than in 48-h-old piglets.

KEY WORDS: • medium-chain triglyceride • odd-chain triglyceride • neonates • piglets • hepatocyte

¹ This research was supported in part by funds from the College of Agriculture and Life Sciences at the University of Wisconsin, Madison, WI and by grants from the National Pork Producers Council and the United States Department of Agriculture (88-37242-4109).

² An abstract of this research was presented at the 1990 meeting of the Federation of American Societies for Experimental Biology. [Odle, J., Benevenga, N. J. & Crenshaw, T. D. (1990) Metabolism of medium- and long-chain fatty acids by isolated hepatocytes from small-for-gestational-age and appropriate-for-gestational-age piglets. FASEB J. 4: A658 (abs. 2271)].

³ Present address: Department of Animal Sciences, University of Illinois, Urbana, IL 61801.

Manuscript received 29 March 1990. Revision accepted 5 October 1990.

This article has been cited by other articles:

				P. L. Peffer, X. Lin, and J. Odle Hepatic {beta}-oxidation and carnitine palmitoyltransferase I in neonatal pigs after dietary treatments of clofibric acid, isoproterenol, and medium-chain triglycerides Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1518 - R1524. [Abstract] [Full Text] [PDF]