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Endotoxin and Lipid Peroxidation in Vivo in Selenium- and Vitamin E-Deficient and -Adequate Rats^1,2,

Departments of Biochemistry, Meat and Animal Science and Nutritional Sciences, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706

The effect of Salmonella typhimurium endotoxin injected intraperitoneally (0.5 mg/kg body weight) on lipid peroxidation in vivo was assessed. Peroxidation was monitored by measuring ethane production, an autoxidation product of (n-3) unsaturated fatty acids. Weanling rats were fed a selenium- and vitamin E-deficient basal diet or one supplemented with 0.2 mg Se/kg and/or 200 mg vitamin E/kg. After 11 to 13 wk of feeding, ethane production was tripled in LPS-treated Se- and vitamin E-deficient rats compared to saline-treated deficient rats. In both doubly deficient and adequate rats, LPS increased ethane production, but it did so to a greater extent in Se- and vitamin E-deficient rats. Dietary Se or vitamin E supplementation alone significantly reduced ethane production from LPS-treated rats. Vitamin E was more protective than Se against LPS-induced lipid peroxidation. Escherichia coli and Salmonella minnesota LPS also increased ethane production in Se- and vitamin E-deficient rats. These results show that low doses of LPS stimulate lipid peroxidation in vivo in Se- and vitamin E-deficient rats.

KEY WORDS: • endotoxin • lipid peroxidation • selenium • vitamin E • rats

¹ A preliminary report of these experiments, Effect of endotoxin on lipid peroxidation in vivo in selenium and vitamin E deficient rats, was presented at the meetings of the Federation of American Societies for Experimental Biology, St. Louis, MO [Sword, J. T., Pope, A. L. & Hoekstra, W. G. (1986) Effect of endotoxin on lipid peroxidation in vivo in selenium and vitamin E deficient rats. Fed. Proc. 45: 475 (abs. 1871)].

² This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison.

³ To whom correspondence should be addressed: Department of Biochemistry, University of Wisconsin-Madison, 420 Henry Mall, Madison, WI 53706.

Manuscript received 12 March 1990. Revision accepted 18 September 1990.