Journal of Nutrition LabDiet, Your World of Nutritional Answers

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murai, T.
Right arrow Articles by Ogata, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murai, T.
Right arrow Articles by Ogata, H.

Changes in Free Amino Acid Levels in Various Tissues of Common Carp in Response to Insulin Injection Followed by Force-Feeding an Amino Acid Diet

Takeshi Murai and Hiroshi Ogata

Nutrition Section, National Research Institute of Aquaculture (Inland Station), Tamaki, Mie 519-04 Japan

The effects of a single injection of porcine insulin on free amino acid levels in plasma, erythrocytes, hepatopancreas and skeletal muscle were simultaneously monitored in common carp; the fish were force-fed a complete diet composed of crystalline amino acids as the sole protein precursors (amino acid diet) to study the dynamics of amino acid metabolism. The force-feeding of the amino acid diet caused surges in the concentrations of almost all amino acids in fish injected with saline, and amino acid levels reached peaks within 1 h in plasma as well as in hepatopancreas. It took more than 2.5 h for most amino acids to reach maximum levels in erythrocytes and skeletal muscle of the same fish. The injection of insulin stimulated drastic reductions of free amino acid levels in the plasma. At the same time, it facilitated reduction of free amino acid levels without elevating glutamine and ammonia levels in erythrocytes, hepatopancreas or skeletal muscle. These results suggest that exogenous insulin accelerated assimilation of dietary free amino acids and their deposition in these tissues.

KEY WORDS: • free amino acids • amino acid diet • insulin injection • amino acid metabolism • common carp

Manuscript received 31 May 1989. Revision accepted 1 February 1990.






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 1990 by American Society for Nutrition