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Kinetics of Cell-Mediated Cytotoxicity in Mice Fed Diets of Various Fat Contents¹

Nutrition and Metabolism Section, College of Medicine, University of Illinois, Urbana, IL 61801

We previously reported lower mitogen-induced blastogenic and cytotoxic activity of splenocytes from C3H/OUJ female mice fed 20% soybean oil (SBO) in their diet compared to those fed 5% SBO. The present study examined the kinetics of cell-mediated cytotoxicity using the same animal model and dietary treatments. Kinetic parameters were determined by analyzing the lytic efficiency of splenocytes cultured for various times with several concentrations of radiolabeled P815 mastocytoma cells. The apparent avidity constant (K_1/2) of the reaction was not changed by dietary SBO intake (1.0 ± 0.2 x 10⁵ cells for 20% SBO versus 1.3 ± 0.3 x 10⁵ cells for 5% SBO). However, the maximum velocity (V_max) of the reaction for splenocytes from mice fed 20% SBO was significantly lower than that for splenocytes from mice fed 5% SBO (1.4 ± 0.2 x 10⁴ cells/h for 20% SBO versus 2.3 ± 0.4 x 10⁴ cells/h for 5% SBO, p < 0.05). The evidence indicates that the rate of target cell lysis, but not the avidity of the lymphocytes for the target cell antigen, was altered by increasing dietary SBO concentration.

KEY WORDS: • dietary fat • cell-mediated cytotoxicity • mice • splenocytes

¹ This research was supported by the National Dairy Promotion and Research Board and by National Institutes of Health grant nos. CA29629 and CA23326.

² Current address: Department of Pathology, University of Chicago, Chicago, IL 60637.

³ To whom reprint requests should be sent.

Manuscript received 21 July 1989. Revision accepted 7 December 1989.