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Effect of Dietary Restriction on the Age-Dependent Changes in the Expression of Antioxidant Enzymes in Rat Liver¹

Department of Biological Sciences ^* Department of Chemistry, Illinois State University, Normal, IL 61761

The effects of aging and dietary restriction on the expression of several enzymes (superoxide dismutase, catalase and glutathione peroxidase) that are involved in free radical detoxification were studied in liver tissue from male Fischer F344 rats. The expression (i.e., activities and mRNA levels) of superoxide dismutase (Cu-Zn) and catalase decreased with age in liver. Dietary restriction (40% restriction of energy intake) increased the activities of superoxide dismutase (24 to 38%) and catalase (64 to 75%) in liver at 21 and 28 mo of age. Glutathione peroxidase activity in liver of diet-restricted rats was significantly higher (37%) at 28 mo of age than that of rats fed ad libitum. The age-related changes in the relative levels of mRNA for superoxide dismutase, catalase and glutathione peroxidase paralleled the changes in the activities of these enzymes in rats fed ad libitum or rats fed the restricted diet. Thus, the changes in the activities of superoxide dismutase, catalase and glutathione peroxidase with age and dietary restriction appear to arise from changes in the levels of mRNAs coding for these enzymes. Free radical damage, as measured by thiobarbituric acid-reactive material and lipofuscin accumulation, was lower in diet-restricted rats than in rats fed ad libitum.

KEY WORDS: • aging • superoxide dismutase • catalase • glutathione peroxidase • rats

¹ This research was supported by grant AG01548 from the National Institute on Aging, by a grant-in-aid of research from Sigma Xi, the Scientific Research Society and by a research grant from the Beta Lambda Chapter of the Phi Sigma Society.

Manuscript received 23 August 1989. Revision accepted 19 January 1990.

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