QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chang, S.-C. Articles by Korc, M. Search for Related Content PubMed PubMed Citation Articles by Chang, S.-C. Articles by Korc, M.

Effects of Dietary Manganese Deficiency on Rat Pancreatic Amylase mRNA Levels^{1, 2,}

Departments of Nutrition and Food Science, University of Arizona, Tucson, AZ 85721 ^* Departments of Medicine and Biochemistry, University of California, Irvine, CA 92717

Manganese deficiency is associated with increased pancreatic amylase activity in rats. The present study investigated whether this increase in amylase activity is associated with increased pancreatic amylase messenger RNA (mRNA) levels. Weanling rats were fed a high carbohydrate diet containing either 40 µg Mn/g (control) or 0.5 µg Mn/g (deficient) for 4 to 8 wk. Mn deficiency was confirmed by determining hepatic Mn content, which was significantly lower in Mn-deficient rats than in controls. Pancreatic RNA from both groups of rats was hybridized with ³²P-labeled complementary DNAs for amylase and trypsinogen. Amylase mRNA levels were increased in rats fed Mn-deficient diets for both 4 wk (200%) and 8 wk (250%) when compared with respective control levels. In contrast, Mn deficiency was not associated with alterations in trypsinogen mRNA levels. Serum levels of insulin and corticosterone, hormones known to increase pancreatic amylase mRNA, were not affected by Mn deficiency. These observations suggest that Mn may participate in the regulation of amylase gene expression in a manner that is independent of insulin and corticosterone.

KEY WORDS: • manganese • amylase mRNA • trypsinogen mRNA • rats

¹ This research was supported in part by National Institutes of Health grant DK-32561 to M. Korc.

² Part of a thesis submitted by S.-C. Chang to the Graduate College of the University of Arizona and the Graduate Group on Nutritional Sciences in partial fulfillment of the requirements for the M.S. degree.

³ To whom reprint requests should be addressed.

Manuscript received 16 January 1990. Revision accepted 18 April 1990.