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Journal of Nutrition Vol. 120 No. 1 January 1990, pp. 71-80
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Nitrous Oxide Provokes Changes in Folylpenta- and Hexaglutamates1

Tom Brody2 and E. L. R. Stokstad3

Department of Nutritional Sciences, University of California, Berkeley, CA 94720

Liver folates isolated from rats fed a control diet consisted of H4PteGlu5 (44% of total) and 5-methyl-H4PteGlu5 (19%) with smaller amounts of 10-formyl-H4PteGlu5 (13%), H4PteGlu6 (13%), 10-formyl-H4PteGlu6 (4%) and 5-methyl-H4PteGlu6 (2%). Folates from methionine-deficient rats contained more nearly equal amounts, on a molar basis, of folylpenta- and hexaglutamates, and hence these were more suitable subjects for comparing the metabolism of these compounds in vivo. Livers from methionine-deficient rats contained H4PteGlu5 (30%), 5-methyl-H4PteGlu5 (13%), 10-formyl-H4PteGlu5 (10%), H4PteGlu6 (22%), 5-methyl-H4PteGlu6 (9%) and 10-formyl-H4PteGlu6 (11%). With exposure to N2O, 5-methyl-H4PteGlu5 and 6 increased to 27% and 13%, respectively, whereas H4PteGlu5 and 6 decreased to 20% and 17%, respectively. Nitrous oxide (N2O) perturbed both the penta- and hexaglutamates; the effect was somewhat more pronounced with the pentaglutamates. The partial depletion in tissue H4PteGlun with N2O treatment helps explain the concomitant inhibition of the oxidation of [ring-14C]histidine, an event dependent on tetrahydrofolates.

KEY WORDS: • folylpolyglutamates • nitrous oxide • histidine • methionine deficiency • male rats

1 This work was supported by U.S. Public Health Service Grant No. AM-08171 from the National Institutes of Health.

2 Present address: Dept. of Food Science and Human Nutrition, Univ. of Hawaii at Manoa, Honolulu, HI 96822.

3 To whom correspondence should be addressed at: Dept. of Nutritional Sciences, 119 Morgan Hall, Univ. of California, Berkeley, CA 94720.

Manuscript received 26 April 1989. Revision accepted 11 September 1989.






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