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The Use of a 10-Day Period for the Assay of Vitamin B by Rat Growth Technic¹

Six Figures

Department of Pediatrics, The University of Chicago

A short period technic for vitamin B assays has been developed through a study of the factors producing variability in results.

The amount of growth of the rat during 10 days was found to be a valid criterion of the quantity of vitamin B in the dose material if the following precautions had been observed.

1. The basal ration contained autoclaved whey in preference to autoclaved yeast, and sucrose instead of dextrin.

2. The rats weighed about 60 gm. when started on depletion.

3. The amount of dose was controlled each day to keep the rate of gain of the rats between 1 and 2 gm. per day.

The quantity of vitamin B causing 1 gm. of gain, when the rate of gain has been limited to 1 to 2 gm. per day, is suggested as a unit since it is comparable to the minimum curative dose defined by Ammerman and Waterman.

Manuscript received 11 July 1936.