Journal of Nutrition

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Journal of Nutrition Vol. 119 No. 9 September 1989, pp. 1311-1317
Copyright © 1989 by American Society for Nutrition
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Lack of an Influence of Dietary Fat on Murine Natural Killer Cell Activity1

Kent L. Erickson and Leslie A. Schumacher

Department of Human Anatomy, University of California, School of Medicine, Davis, CA 95616

The influence of the degree of saturation and the concentration of dietary fat on murine natural killer (NK) cell activity was investigated using C3H/HeN and C57BL/6N mice. Mice were fed purified diets containing either 0% fat (fat free), 5 or 20% safflower oil or 5 or 20% palm oil. Safflower oil and palm oil were used because they are comparable in carbon chain length but vary in the amount of linoleic acid [18:2(n-6)]. Cytotoxicity of splenic NK cells from mice stimulated or unstimulated by the interferon inducer poly I:C was measured against either the YAC-1 lymphoma or line 168 mammary tumor targets. The number of asialo GM1 + cells was not influenced by concentration or degree of saturation of dietary fat. Generally, dietary fat had no consistent influence on NK cell cytotoxicity of spleen cells from either the high responder C3H/HeN mice or the moderate responder C57BL/6N mice against either tumor target. The level of 18:2(n-6) in NK cell-enriched splenic lymphocytes increased with greater levels of dietary safflower oil. Nevertheless, there appeared to be no correlation between lymphocyte fatty acid composition and NK cell cytotoxic capabilities. Therefore, the concentration of dietary safflower or palm oil, and thus 18:2 (n-6), did not appear to effect the number of activity of murine NK cells.

KEY WORDS: • dietary fat • natural killer (NK) cell • tumor cytotoxicity • cell-mediated immunity • spleen • safflower oil • palm oil • poly I:C • mice

1 This work was supported by a grant from the National Live Stock and Meat Board, Chicago, IL.

Manuscript received 11 April 1988. Revision accepted 24 April 1989.






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