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Influence of Dietary Methionine on the Metabolism of Selenomethionine in Rats^1,2,

Department of Agricultural Chemistry, Oregon State University, Corvallis, OR 97331

To determine the influence of methionine on selenomethionine (SeMet) metabolism, weanling male rats were fed for 8 wk a basal diet marginally deficient in sulfur amino acids, containing 2.0 µg selenium (Se)/g as DL-SeMet and supplemented with 0, 0.3, 0.6 or 1.2% DL-methionine. Increased dietary methionine caused decreased selenium deposition in all tissues examined but increased glutathione peroxidase (GSHPx, EC 1.11.1.9) activity in testes, liver and lungs. A positive correlation was found between dietary methionine and the calculated percentage of selenium associated with GSHPx. In a second experiment, ⁷⁵SeMet was injected into weanling male rats which had been fed the basal diet containing 2.0 µg selenium as DL-SeMet with or without the addition of 1.0% methionine. The selenoamino acid content of tissues and the distribution of ⁷⁵Se in erythrocyte proteins were determined. In comparison to the rats fed the basal diet without added methionine, significantly more ⁷⁵Se-selenocysteine was found in liver and muscle, more ⁷⁵Se was found in erythrocyte GSHPx and less ⁷⁵Se was found in erythrocyte hemoglobin of rats fed 1.0% methionine. These data suggest that methionine diverts SeMet from incorporation into general proteins and enhances its conversion to selenocysteine for specific selenium-requiring proteins, such as GSHPx.

KEY WORDS: • dietary methionine • selenomethionine metabolism • tissue selenium • glutathione peroxidase • rat • hemoglobin • selenocysteine

¹ Supported by Public Health Service Research Grant DK 38306 from National Institute of Diabetes and Digestive and Kidney Diseases.

² Published with the approval of Oregon State University Agricultural Experiment Station as Technical Paper Number 8425.

Manuscript received 9 February 1988. Revision accepted 8 March 1989.

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