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Synthesis of Amylase by Cultured Rat Pancreatic Acinar Cells: Effects of Antecedent Diet^1,2,3,

Department of Nutrition and Food Science ^* Nutritional Sciences Program, University of Arizona, Tucson, AZ 85721

Amylase synthesis in cultured pancreatic acinar cells was determined using an affinity adsorbent, -glucohydrolase inhibitor—Sepharose 4B. This adsorbent exhibited a consistent binding capacity and was specific for amylase. To assess the effects of antecedent diet on amylase synthesis during culture, acinar cells from rats fed high fat (HF) or high carbohydrate (HC) diets for 7 d were cultured for 1–48 h in serum-free medium. Amylase activity remained significantly higher in cells from rats fed the HC diet than in cells from rats fed the HF diet through 24 h in culture, despite an overall decrease with time in culture. The relative synthesis of amylase [([³H]phenylalanine amylase/[³H]phenylalanine total protein) x 100] was also significantly higher in cells from HC-fed rats than in cells from HF-fed rats at isolation and remained higher during culture. The results demonstrate that these cultured acinar cells synthesize amylase in vitro and that the effect of diet on amylase activity and relative synthesis persists during culture.

KEY WORDS: • amylase • exocrine pancreas • diet adaptation • high fat diet • high carbohydrate diet • cultured pancreatic acinar cells

¹ Presented in part at the 71st Annual Meeting of the Federation of American Societies for Experimental Biology. [Zenger, J. D. & Brannon, P. M. (1987) The effects of diet on amylase synthesis in cultured pancreatic acinar cells. Fed. Proc. 46: 1494 (abs.)].

² Supported in part by the Hatch Project 174076 of the Arizona Agriculture Experiment Station and National Institutes of Health Research Grant AM32690 from the National Institute of Diabetes, Digestive and Kidney Diseases.

³ Part of a thesis submitted by J. D. Justice to the Graduate College of the University of Arizona in partial fulfillment of the requirements for the M.S. degree for the Nutritional Sciences Program.

⁴ To whom requests for reprints should be addressed.

Manuscript received 10 November 1988. Revision accepted 17 January 1989.