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Journal of Nutrition Vol. 119 No. 4 April 1989, pp. 547-552
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Dietary Protein Enhanced Mammary Ornithine Decarboxylase Activity and Tumorigenesis in Rats1

E. J. Hawrylewicz2, Carl K. Moshovitis, Christopher Reger and Janet Madell

Department of Research, Mercy Hospital and Medical Center, Chicago, IL 60616

Sprague-Dawley rats (F-1) were fed a normal protein (19% casein, NP) or high protein (33% casein, HP) isoenergetic diet containing 15% corn oil prior to conception. Female pups (F-2) were also fed the maternal diet after weaning. At 7 wk of age, before saline or N-nitrosomethylurea (NMU) treatment, omithine decarboxylase (ODC, EC 4.1.1.17) activity in mammary epithelium and liver tissue was significantly higher in the HP group than in the NP group. Eight weeks after saline treatment ODC activity in mammary tissue decreased in both groups, but remained significantly higher in the HP group. NMU treatment caused a sixfold increase in ODC activity in the mammary tissue in the HP group and a significantly lower response in the NP group. Liver ODC activity had a minimal response to NMU treatment. Changing from the HP to the NP diet 4 wk after NMU treatment reduced mammary ODC induction response but not tumor burden; changing from the NP diet to HP diet produced no change in ODC activity or tumor burden. Mammary tumor burden was positively related to dietary protein and mammary epithelium ODC activity prior to and following NMU treatment.

KEY WORDS: • ornithine decarboxylase • mammary tumorigenesis • dietary protein • mammary development • liver • N-nitrosomethylurea

1 Supported by Grant CA-35573 awarded by the National Cancer Institute, National Institutes of Health, Bethesda, MD.

2 To whom requests for reprints should be addressed at: Department of Research, Mercy Hospital & Medical Center, Stevenson Exp. at King Drive, Chicago, IL 60616.

Manuscript received 26 April 1988. Revision accepted 9 January 1989.






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