QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gaskins, H. R. Articles by Hausman, G. J. Search for Related Content PubMed PubMed Citation Articles by Gaskins, H. R. Articles by Hausman, G. J.

Evidence for Abnormal Prostaglandin Synthesis in Obese Zucker Rat Adipose Cell Cultures¹

H. Rex Gaskins, Dorothy B. Hausman, Roy J. Martin and Gary J. Hausman

Department of Foods and Nutrition, University of Georgia, Athens, GA 30602 Richard B. Russell Agricultural Research Center, United States Department of Agriculture, Athens, GA 30613

Prostaglandin E₂ (PGE₂) is synthesized in adipose tissue and acts locally to inhibit lipolysis. This study examined PGE₂ synthesis by presumptive and mature adipocytes isolated from lean and obese Zucker rats. Isoproterenol-stimulated lipolysis was greater in short-term incubations of mature adipocytes isolated from lean rats than in those from obese rats in terms of both sensitivity (lean 10^-7 M vs. obese 10^-6M) and magnitude of response (five-fold greater in lean at 10^-6M). Prostaglandin E₂ synthesis was dose-dependent in isoproterenol-stimulated adipocytes from lean rats, while PGE₂ synthesis in adipocytes from obese rats was not altered, regardless of treatment. Primary cell cultures of presumptive adipocytes from both phenotypes released PGE₂ in response to lipolytic compounds, however, cultures from obese rats had lower PGE₂ release rates than cultures from lean rats. In addition, compared to cultures derived from lean rats, a greater degree of adipocyte differentiation in cultures from obese rats was associated with lower basal PGE₂ synthesis. These results suggest that an impairment in the ability of adipocytes isolated from obese Zucker rats to enzymatically synthesize PGE₂ accompanies maturation.

KEY WORDS: • Zucker (fa/fa) rat • prostaglandin E₂ • adipocyte differentiation

¹ This work was supported by National Institutes of Health research grant HD22226 (R.J.M.)

Manuscript received 20 May 1988. Revision accepted 8 November 1988.

This article has been cited by other articles:

				I. Nitz, E. Fisher, H. Grallert, Y. Li, C. Gieger, D. Rubin, H. Boeing, J. Spranger, I. Lindner, S. Schreiber, et al. Association of Prostaglandin E Synthase 2 (PTGES2) Arg298His Polymorphism with Type 2 Diabetes in Two German Study Populations J. Clin. Endocrinol. Metab., August 1, 2007; 92(8): 3183 - 3188. [Abstract] [Full Text] [PDF]