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Journal of Nutrition Vol. 119 No. 2 February 1989, pp. 152-160
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Disparate Effects of Dietary Fatty Acids on Activity of 5'-Nucleotidase of Rat Liver Plasma Membrane1

M. E. Zuniga, B. R. Lokesh and J. E. Kinsella

Lipid Research Laboratory, Institute of Food Science, Cornell University, Ithaca, NY 14853

The effects of incorporation of dietary n-3 polyunsaturated fatty acids (PUFA) into rat liver plasma membrane on the activity of 5'-nucleotidase (EC 3.1.3.5) was studied. The membrane phospholipids from rats fed a diet containing 10% by weight menhaden oil (MO) for 3 wk contained more n-3 PUFA and less n-6 PUFA in phospholipids classes, i.e., 24% and 65% less linoleic and arachidonic acid in phosphatidylcholine, than in rats fed 10% coconut oil (CNO) diets. The specific activity of 5'-nucleotidase in n-3 PUFA-enriched hepatic plasma membranes was 1.6- to 2-fold higher than that in rats fed CNO or corn oil (CO). Lineweaver-Burk plots for 5'-nucleotidase in liver plasma membranes isolated from rats fed MO and CNO showed no significant differences in Km values but the Vmax was increased by 67% in MO-fed rats. Arrhenius plots showed a break point in 5'-nucleotidase activity at 28.3°C and 30.8°C in plasma membranes from MO- and CNO-fed rats, respectively. The implications of this in the generation of adenosine and its possible impact on physiological functions are discussed.

KEY WORDS: • 5'-nucleotidase • rat liver plasma membranes • saturated fat • n-6 and n-3 polyunsaturated fatty acids • eicosapentaenoic acid • docosahexaenoic acid • adenosine

1 Supported in part by U.S. Department of Agriculture Nutrition Grant No. 86-CRCR-1-2186 and by the New York SeaGrant Program.

Manuscript received 8 February 1988. Revision accepted 26 September 1988.






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