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Effects of Vitamin B-6 Deficiency on Selenium Metabolism in the Rat^{1 ,2}

Department of Agricultural Chemistry, Oregon State University, Corvallis, OR 97331

Rats were fed for 23 d diets adequate or deficient in vitamin B-6 and containing selenium as either sodium selenite, selenocysteine (SeCys) or selenomethionine (SeMet). They were then injected with ⁷⁵Se of the same chemical form and killed 2 d later. Tissue deposition of stable and radiotracer selenium and the activity of glutathione peroxidase (GSHPx) were used to assess selenium utilization. Erythrocyte levels of selenium and GSHPx were lower in vitamin B-6—deficient animals for all forms of selenium; however, ⁷⁵Se deposition in erythrocytes was not affected by vitamin B-6 status. The activities of cystathionine lyase, aspartate aminotransferase and selenocysteine lyase were lower in livers of vitamin B-6—deficient rats than in vitamin B-6—supplemented rats. The proportion of liver and kidney ⁷⁵Se soluble in 5% trichloroacetic acid and 0.1 M 2-mercaptoethanol was consistently lower in vitamin B-6—deficient animals, but cation-exchange chromatography of tissue extracts did not identify a specific low-molecular-weight species. Tissue retention of ⁷⁵Se provided as SeMet was increased in vitamin B-6—deficient animals, but the proportion of ⁷⁵Se retained in muscle and liver as SeCys was significantly reduced. These findings suggest that the conversion of SeMet to a form available for GSHPx synthesis is reduced by vitamin B-6 deficiency.

KEY WORDS: • vitamin B-6 deficiency • selenomethionine • selenocysteine • selenite • selenocysteine lyase • cystathionine lyase • glutathione peroxidase • weanling male rats

¹ Supported by Public Health Service Research Grant DK 38306 from the National Institute of Diabetes and Digestive and Kidney Diseases.

² Published with the approval of Oregon State University Agricultural Experiment Station as Technical Paper no. 8643.

Manuscript received 19 October 1988. Revision accepted 1 August 1989.

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				J. Ortuno, G. Ros, M.J. Periago, C. Martinez, and G. Lopez Biodisponibilidad del selenio y metodos de evaluacion/Selenium bioavailability and methods of evaluation Food Science and Technology International, January 1, 1996; 2(3): 135 - 150. [PDF]