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Effect of Dietary Proline on Proline Metabolism in the Neonatal Pig^{1 ,2}

Susan E. Samuels^*, Henk L. M. Aarts^* and Ronald O. Ball^*,,3

^* Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 1A8

The effect of two dietary concentrations of proline (10.3 and 15.8 g/kg) on proline-metabolizing enzymes [pyrroline-5-carboxylate (P5C) reductase and proline oxidase], plasma and tissue free proline concentrations and growth were investigated in the 2- to 13-d-old pig. Diet had no effect on growth or enzyme activity. Diet had a significant (P < 0.05) effect on the concentration of free proline in plasma, liver, intestine and muscle, but no effect in kidney. These data suggest that the magnitude and pattern of change of P5C reductase activity is not influenced by the concentration of proline in the diet. The lower plasma and tissue free proline concentrations in the piglets fed the basal diet compared with piglets fed the proline-supplemented diet and the lack of effect of diet on enzyme activity suggest there was inadequate proline in the basal diet, and those piglets were unable to increase proline synthesis to maintain normal proline concentrations.

KEY WORDS: • proline requirement • pyrroline-5-carboxylate reductase • proline oxidase • pig

¹ Financial assistance from the Natural Sciences and Engineering Research Council of Canada and the Ontario Ministry of Agriculture and Food.

² For related article, see pp. 1999–2004 of this issue.

³ To whom requests for reprints should be sent.

Manuscript received 3 June 1988. Revision accepted 18 April 1989.

This article has been cited by other articles:

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