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Identification of Transferrin as the Major Plasma Carrier Protein for Manganese Introduced Orally or Intravenously or After In Vitro Addition in the Rat¹

Lena Davidsson^*, Bo Lönnerdal, Brittmarie Sandström^*, Clemens Kunz and Carl L. Keen

^* Department of Clinical Nutrition, University of Gothenburg, Sahlgrenska Hospital, S-413 45 Göteborg, Sweden Department of Nutrition, University of California, Davis, CA 95616

It is known that the metabolic handling of manganese (Mn) introduced via the diet or by intravenous injection is quite different. We hypothesized that this difference could be due in part to different proteins carrying Mn in plasma that could affect tissue uptake and retention. To test this idea, ⁵⁴Mn was administered orally or intravenously to rats, and blood samples were taken by cardiac puncture at various time points postdosing. Plasma proteins were separated using fast protein liquid chromatography with a combination of anion exchange and gel filtration columns. Using these methods, independent of the route of ⁵⁴Mn administration, transferrin was identified as the major Mn-binding protein in plasma. The identity was further confirmed by SDS-polyacrylamide gradient gel electrophoresis and Western blotting. These results conclusively show that ⁵⁴Mn in plasma is carried by transferrin, regardless of route of administration and time postdosing.

KEY WORDS: • manganese • transferrin • plasma protein • fast protein liquid chromatography • rat

¹ Research supported by the U.S. Department of Agriculture, USDA Grant No 85-CRCR-1-1582.

Manuscript received 7 November 1988. Revision accepted 8 May 1989.

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