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Effect of Dietary Taurine on Renal Taurine Transport by Proximal Tubule Brush Border Membrane Vesicles in the Kitten^1,2,

Taesun Park^*, Quinton R. Rogers^*, James G. Morris and Russell W. Chesney

^* Departments of ^* Physiological Sciences and Animal Science, University of California, Davis, CA 95616 Department of Pediatrics, University of Tenesse, Le Bonheur Children's Medical Center Memphis, TN 38103

Renal adaptation of the kitten to altered dietary taurine intake was assessed using proximal tubule brush border membrane (BBM) vesicles. Three groups of kittens were adapted to purified diets containing 43.5% soy protein that were either taurine-free (0T) or contained 0.15% taurine (NT) or 1.0% taurine (HT). The plasma taurine concentration of the kittens fed 0T decreased from 104 ± 25 µM to 16 ± 5 µM and 1.7 ± 0.5 µM in 1 and 6 wk, respectively. Feeding HT increased plasma taurine concentration to 350 ± 116 µM in 1 wk. Compared to NT, taurine accumulation by BBM vesicles was significantly elevated after 4 wk of feeding 0T and decreased after 2 wk or less of feeding HT (P < 0.05). Maximum renal adaptation occurred by 6 wk of feeding 0T (206% increase in taurine uptake/15 s compared to NT) and by 2 wk or less of feeding HT (43% decrease in taurine uptake/15 s compared to NT). Evaluation of transport kinetics using renal cortex from groups of four kittens (16 determinations) fed NT, 0T (12 wk) or HT (10 wk) revealed a V_max of 55 ± 10, 123 ± 24 or 39 ± 7 pmol · mg protein^-1 · 10 s^-1 and a K_m of 32 ± 7, 16 ± 2 or 37 ± 8 µM, respectively. The differences in V_max and K_m were significant between NT and 0T (P < 0.05), but not significant between NT and HT (P > 0.05). Our results suggest that renal adaptation of the kitten to changes in dietary taurine occurs with modifications of both V_max and K_m of the taurine transport system.

KEY WORDS: • proximal tubule brush border membrane vesicles • renal taurine transport • renal adaptation • kitten

¹ Presented in part at the 72nd Annual Meeting of the Federation of the American Societies for Experimental Biology, held in Las Vegas, NV, May 5, 1988.

² Supported in part by U.S. Public Health Service Grant DK 37223.

Manuscript received 7 October 1988. Revision accepted 26 May 1989.

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