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Journal of Nutrition Vol. 118 No. 9 September 1988, pp. 1116-1119
Copyright © 1988 by American Society for Nutrition
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Choline-Deficient Diet Increases Z Protein Concentration in Rat Liver1

Asim K. Dutta-Roy, Manh V. Trinh, Thomas F. Sullivan and Dietmar V. Trulzsch2

Veterans Administration Medical Center and Department of Medicine, Wright State University School of Medicine, Dayton, OH 45401-0927

Feeding rats a diet deficient in choline results in fatty liver within 1 d. We studied the effect of shortterm (1–3 d) choline deficiency on rat liver Z protein (fatty acid-binding protein). Groups of three female Sprague-Dawley rats were fed ad libitum a purified diet lacking choline and L-methionine or were supplemented with 0.2% choline chloride and 0.82% L-methionine. Animals were killed after 1, 2 or 3 d of consuming control or experimental diets and hepatic Z protein was prepared. Z protein in livers from experimental and control rats was estimated with the fluorescent probe dansylamino undecanoic acid. The corresponding fatty acid-binding activity was also determined. One day of choline-deficient diet increased Z protein concentration threefold, reaching a plateau on the second and third day. Fatty acid-binding activity of Z protein remained unchanged.

KEY WORDS: • choline-deficient diet • Z protein • fatty acid-binding protein • fatty acid-binding activity • rat liver

1 This work was supported in part by the Veterans Administration Medical Research Service and the American Heart Association, Miami Valley Chapter, Dayton, OH.

2 To whom reprint requests should be sent, at the Department of Medicine, Wright State University, P.O. Box 927, Dayton, Ohio 45401-0927.

Manuscript received 18 December 1987. Revision accepted 24 May 1988.






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