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Journal of Nutrition Vol. 118 No. 8 August 1988, pp. 987-994
Copyright © 1988 by American Society for Nutrition
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Effect of Dietary Zinc Deficiency and the Associated Drop in Voluntary Food Intake on Rat Erythrocyte Membrane Polyamines

Ross A. Avery and William J. Bettger

Department of Nutritional Sciences, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1

The effect of dietary zinc deficiency in the rat on the free (noncovalently bound) polyamine concentrations in erythrocyte membranes, in erythrocyte cytosol and in the blood plasma were determined. Weanling male Wistar rats were fed an egg white—based diet containing < 1.0 mg Zn/kg diet for 3 wk ad libitum. Control rats were either pair-fed or ad libitum—fed the basal diet supplemented with 100 mg Zn/kg diet. Tissue fractions were extracted with 0.2 M perchloric acid and polyamines were measured in the soluble fraction by dansylation, thin-layer chromatography and fluorescence spectrophotometry of the isolated dansylated polyamines. The depressed food intake associated with dietary zinc deficiency caused significantly (P < 0.05) lower plasma spermidine, erythrocyte cytosol putrescine, spermidine and spermine and erythrocyte membrane spermidine concentrations. In addition, the depressed food intake caused significantly lower spermidine-spermine ratios in the erythrocyte membrane and cytosol and in blood plasma. Dietary zinc deficiency per se caused significantly (P < 0.05) higher plasma spermidine and spermine and erythrocyte membrane spermine concentrations and significantly lower spermidine-spermine ratios in erythrocyte membrane and cytosol than in pair-fed, zinc-adequate controls.

KEY WORDS: • zinc • polyamines • erythrocyte membrane

Manuscript received 28 December 1987. Revision accepted 17 March 1988.






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