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Effect of Selenium Repletion on Glutathione Peroxidase Protein Level in Rat Liver¹

Department of Nutrition and Food Science and the Nutritional Sciences Program, University of Arizona, Tucson, AZ 85721

We have previously reported that liver glutathione peroxidase (GSH-Px, EC 1.11.1.9) protein level and activity decrease exponentially during Se deficiency. To determine the effect of Se repletion on these parameters, Se-deficient rats were repleted with 0.1 or 0.5 mg Se/kg diet as Na₂SeO₃ in a 30% torula yeast—based diet and were killed 0, 1, 2, 3, 5, 7 or 14 d later. GSH-Px protein was quantitated using anti-GSH-Px antibodies. Dietary repletion with 0.5 mg Se/kg diet increased GSH-Px protein and activity significantly (P < 0.05) after 1 d. After 5 d for GSH-Px protein and 7 d for activity the rate of increase slowed, and at d 14 neither GSH-Px protein nor activity was significantly different from that of Se-adequate rats. Repletion with 0.1 mg Se/kg diet did not significantly increase GSH-Px protein or activity until 14 d. To examine the short-term effect of Se repletion, Se-deficient rats were injected intravenously with 15 or 60 µg Se as Na₂SeO₃ and killed 1, 3, 6, 12 or 24 h later. Only rats injected with 60 µg Se and killed 24 h later had a significant increase in GSH-Px activity along with a marginally significant increase in GSH-Px protein. These response curves indicate that homeostatic processes control the level of GSH-Px. The lack of an increase in GSH-Px until 24 h after Se administration implies that additional metabolic events after a rise in cellular Se may be necessary prior to an increase in GSH-Px synthesis in Se-deficient rats.

KEY WORDS: • GSH-Px • selenium • antibodies • rat liver

¹ This research was supported in part by the University of Arizona Experiment Station and by grants from the National Institutes of Health (DK 32942) and the Biomedical Research Support Grant Program (S07 RR07002).

Manuscript received 14 December 1987. Revision accepted 3 March 1988.

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