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Effect of Sera from Control and Overfed Rats on Preadipocyte Growth in Culture

Department of Foods and Nutrition, University of Georgia, Athens, GA 30602 ^* U.S. Department of Agriculture—Agricultural Research Service, Richard B. Russell Research Center, Athens, GA 30602

Increased body lipid was induced in 4-wk-old rats through oral gavage. Rats were either fed ad libitum, tube-fed 100% or tube-fed 150% of the ad libitum intake. After 1 wk of tube-feeding animals were killed and sera collected. Sera were used to support the growth of rat stromal-vascular cells in culture. Sera were also analyzed for growth hormone and insulin concentrations. In rats that were tube-fed, levels of adiposity were greater but sera from these rats decreased stromal-vascular and preadipocyte proliferation in culture when compared with sera from ad libitum—fed rats. Sera from tube-fed animals promoted an increase in preadipocyte differentiation as revealed by esterase staining. Results indicate that overfeeding in juvenile rats causes sera changes that support fat cell differentiation but not fat cell hyperplasia.

KEY WORDS: • preadipocytes • overfeeding • proliferation • differentiation • rats

Manuscript received 8 July 1987. Revision accepted 3 March 1988.