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A Modified Relative Dose-Response Assay Employing 3,4-Didehydroretinol (Vitamin A₂) in Rats^1,2,3,

Department of Biochemistry and Biophysics, Iowa State University, Ames, IA 50011

3,4-Didehydroretinol (vitamin A₂, DR, dehydroretinol), a naturally occurring analogue of retinol (vitamin A₁, R), is active in vision, growth and cellular differentiation but is converted to retinol in very small amounts, if at all. When vitamin A-depleted rats were given 500 µg of R acetate, a naturally occurring mixture of 480 µg DR ester and 20 µg R ester or 500 µg DR acetate orally in corn oil, serum levels of all administered retinoids peaked between 3.5 and 5 h and then declined. When an oral dose of 600 µg DR/kg body wt was administered to rats with various liver reserves of vitamin A, the serum ratio of DR to R at 3.5 h was inversely related to the liver reserves of vitamin A below approximately 2 µg/g liver. Because the administration of DR does not affect serum R values, a single blood sample taken at 3.5 h might provide information analogous to that obtained from two blood samples in the conventional relative dose-response method.

KEY WORDS: • 3,4-didehydroretinol • vitamin A₂ • assessing vitamin A status • relative dose response

¹ Presented in part at the Annual Meeting of the Federation of American Societies for Experimental Biology, March 1987, Washington, DC [Tanumihardjo, S. A. & Olson, J. A. (1987) A modified relative dose-response assay employing 3,4-didehydroretinol (vitamin A₂) in rats. Fed. Proc. 46: 1190 (abs.)].

² This work was supported by National Institutes of Health Grant No. DK 32793, by USDA Competitive Research Grant No. 84-CRCR-1-1418 and by the Thrasher Research Fund.

³ Journal Paper No. J-12760 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 2534.

⁴ To whom correspondence and reprint requests should be directed.

Manuscript received 17 August 1987. Revision accepted 6 January 1988.

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