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Incomplete Utilization of Pyridoxine-ß-Glucoside as Vitamin B-6 in the Rat^1,2,

Food Science and Human Nutrition Department, University of Florida, Gainesville, FI 32611

This research was conducted to determine the bioavailability of 5'-O-(ß-D-glucopyranosyl) pyridoxine (PN-glucoside) during chronic administration in a depletion-repletion bioassay. PN-glucoside was found previously to constitute of major portion of the total vitamin B-6 in many foods of plant origin. Following a 14-d depletion period, rats were fed diets containing graded levels of either free pyridoxine (PN) or PN-glucoside for 17 d. Slope ratio analysis of dose-response curves, on the basis of growth and plasma pyridoxal 5-phosphate (PLP) concentration, indicated 10–34% utilization of PN-glucoside relative to the molar response to PN. Erythrocyte aspartate aminotransferase (AspAT) activity and urinary 4-pyridoxic acid concentration were lower and the stimulation of AspAT activity by exogenous PLP was greater for rats fed PN-glucoside than for those fed PN, which indicated reduced vitamin B-6 nutriture in response to PN-glucoside. A constant 7–9% of the ingested PN-glucoside was detected in urine in intact form at all dosage levels. These results provide further evidence of incomplete bioavailability of PN-glucoside and indicate that its extent of utilization is not influenced by its level of dietary intake.

KEY WORDS: • vitamin B-6 • pyridoxine • pyridoxine-ß-glucoside

¹ Supported in part by National Institutes of Health Grant No. DK37431.

² Florida Agricultural Experiment Station Journal Series No. 8606.

³ To whom correspondence should be addressed.

Manuscript received 5 August 1987. Revision accepted 27 October 1987.

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