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Journal of Nutrition Vol. 118 No. 12 December 1988, pp. 1470-1474
Copyright © 1988 by American Society for Nutrition
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In Vivo Utilization of Cystine-Containing Synthetic Short-Chain Peptides after Intravenous Bolus Injection in the Rat1

Peter Stehle, Sabine Albers2, Leonhard Pollack and Peter Fürst

Institute for Biological Chemistry and Nutrition, University of Hohenheim, D-7000 Stuttgart, West Germany

For the first time, in vivo utilization of two highly soluble and stable cystine-containing synthetic short-chain peptides, bis-L-alanyl-L-cystine and bis-glycyl-L-cystine, were investigated in adult rats. Within 5 min after an intravenous bolus, blood samples were drawn (inferior vena cava) and plasma amino acid and peptide levels were determined using RP-HPLC (precolumn derivatization with 5-dimethylaminonaphthalene-1-sulfonylchloride). Both peptides were rapidly cleared from plasma (estimated elimination t1/2:4 min for the glycyl peptide and less than 2 min for the alanyl peptide). The initial high amounts of mono-L-alanyl-L-cystine and mono-glycyl-L-cystine as well as the prompt increase of the constituent free amino acids alanine, glycine and cystine strongly suggest that the peptide disappearance is mainly due to a very fast two-step hydrolysis in the extracellular compartment, presumably catalyzed by soluble and/or plasma membrane-bound peptidases. The observed rapid hydrolysis may serve as first evidence that short-chain peptides with C-terminal cystine residue may represent efficient sources of free cystine in parenteral nutrition.

KEY WORDS: • cystine • peptides • in vivo utilization • parenteral nutrition

1 This study was supported by grants from BMWi (Industrielle Gemeinschaftsforschung, AIF No. 6668) and Pfrimmer and Company, Erlangen, FRG.

2 Present address: ICI Pharma, Plankstadt, FRG.

Manuscript received 19 April 1988. Revision accepted 8 July 1988.






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