QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Walzem, R. L. Articles by Clifford, A. J. Search for Related Content PubMed PubMed Citation Articles by Walzem, R. L. Articles by Clifford, A. J.

Thiamin Absorption is Not Compromised in Folate-Deficient Rats¹

Department of Nutrition, University of California, Davis, CA 95616

Thiamin absorption and excretion were assessed in rats with severe folate deficiency (FD) by determining the fate of oral ³H-labeled and intravenous ¹⁴C-labeled thiamin over a 6-h test period. Thiamin status was evaluated in these same rats by measuring transketolase activity levels of blood before (TKA) and after (TPPE) addition of thiamin pyrophosphate to the incubation mixture of the assay procedure. Two additional experiments assessed active transport of thiamin and the effect of dietary succinylsulfathiazole (SST) on TKA and TPPE in rats with moderate FD. Intestinal absorption in general and thiamin absorption in particular and thiamin status were unaltered in rats with severe FD. Inanition associated with severe FD may impair thiamin status. Thiamin absorption by active transport was not compromised in FD, and dietary succinylsulfathiazole did not affect thiamin status.

KEY WORDS: • folate deficiency • thiamin status • thiamin absorption • rats • amino acid diets

¹ Supported by USPHS Grant Nos. AM-16726 and DK-38637, USDA-CRGO-78-59-2063-0-1-065-1, USDA Regional Research Grant No. W143 and Hatch Grant No. 2850 from California Agricultural Experiment Station.

² Current address: Department of Physiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616.

³ Send reprint requests to: Dr. Andrew J. Clifford, Department of Nutrition, University of California, Davis, CA 95616.

Manuscript received 5 July 1988. Revision accepted 20 July 1988.