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Metabolic Utilization of Pyridoxine-ß-Glucoside in Rats: Influence of Vitamin B-6 Status and Route of Administration^1,2,

Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611

[³H]5'-O-(ß-D-glucopyranosyl) pyridoxine (PN-glucoside) and [¹⁴C]pyridoxine (PN) were administered orally or intraperitoneally to vitamin B-6-adequate or -deficient rats. Analysis of intestinal contents and feces indicated effective intestinal absorption of PN-glucoside relative to PN. There was greater retention of ¹⁴C than ³H in the liver and carcass regardless of the route of administration of the radiolabeled vitamins. There was no major difference in the relative distribution of ³H and ¹⁴C among the vitamin B-6 metabolites in the liver between the treatment groups, and no [³H]PN-glucoside was detected in any of the livers. For all groups, the majority of the ³H administered was detected in the urine within 24 h. Less excretion of both ³H and ¹⁴C in the urine was observed for the deficient rats. There was no major difference in the relative proportion of urinary [³H]PN-glucoside or [³H]4-PA between rats fed or injected with the radiolabeled vitamins. These results indicate that vitamin B-6 status influences the clearance of metabolites derived from PN and PN-glucoside, as well as the clearance of intact PN-glucoside. Vitamin B-6 status, however, has little or no effect on the utilization of PN-glucoside. This study also suggests that the intestine is the primary site of the limited conversion of PN-glucoside to biologically active PN in the rat.

KEY WORDS: • pyridoxine-ß-glucoside • vitamin B-6 • rat • bioavailability

¹ Supported in part by National Institutes of Health Grant DK37481.

² Florida Agricultural Experiment Station Journal Series No. 9321.

³ To whom correspondence should be addressed.

Manuscript received 27 April 1988. Revision accepted 8 July 1988.

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