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Lipoprotein Lipase Activity and Chylomicron Clearance in Rats Fed a High Fat Diet^1,2,

Divisions of Foods and Nutrition and Nutritional Sciences, University of Illinois, Urbana, IL 61801

The relationships of tissue and plasma lipoprotein lipase (LPL) activities to tissue uptake and plasma clearance of ¹⁴C-labeled chylomicron-triglyceride (¹⁴C-CM-TG) were studied in female rats fed isoenergetic and isonitrogenous control (12% kJ from fat) or high fat diets (72% kJ from fat) for 8 wk. Animals fed the high-fat diet had higher levels of fasting plasma triglycerides and lower LPL activities in heart, renal adipose tissue and post-heparin plasma. Changes in LPL activities of skeletal muscles varied among muscles with higher values in the soleus and plantaris (32–61%) and no differences in the gastrocnemius. The lower LPL activity in renal adipose tissue was associated with lower uptake of fatty acids from ¹⁴C-CM-TG by adipose. Fatty-acid uptake from labeled TG was not associated with tissue LPL activity in other tissues. Clearance of ¹⁴C-CM-TG from plasma and the half-lives of ¹⁴C-CM-TG were similar in both dietary groups. These data indicate that tissue and plasma LPL activities are not a direct index of uptake of fatty acids by tissues or clearance of chylomicron triglycerides.

KEY WORDS: • lipoprotein lipase • chylomicrons • triglycerides • high fat feeding

¹ Presented in part at the Federation of American Societies for Experimental Biology annual meeting, Washington, DC, April 1, 1987. Fed. Proc. 46: 1165.

² This research was supported in part by the American Heart Association/Illinois Affiliate.

³ Postgraduate scholar, Natural Sciences and Engineering Research Council of Canada and Charlotte Biester Fellow, College of Agriculture, University of Illinois.

⁴ To whom reprint request should be sent.

Manuscript received 12 November 1987. Revision accepted 6 July 1988.