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Uptake and Release of Adrenal Ascorbic Acid in the Guinea Pig after Injection of ACTH^{1, 2, 3,}

Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853

The effect of a single injection of ACTH (3 KI/100 g body weight) on the distribution of ascorbic acid (AA) and radiolabeled AA in 20 tissues was studied in adult male guinea pigs consuming 500 mg AA/kg diet. Saline- or ACTH-injected animals were simultaneously injected with [1-¹⁴C]AA, and killed at 0.5, 1, 2, 4 and 6 h after injection. There was no significant difference between treatments in the weight of any tissue over the 6-h experimental period. As anticipated, the concentration of AA in the adrenals of animals injected with ACTH was 33% of that of animals injected with saline at 4 h. Unexpectedly, the concentration of radiolabeled AA in the adrenals at 0.5 h after ACTH injection was 172% of that after saline injection. The concentration of radiolabeled AA in the adrenal of the saline-injected animals increased slowly over time to reach a level similar to that of ACTH-injected animals by 6 h. There was no effect of ACTH on the level of AA or uptake in any of the other tissues examined. These results demonstrate that a single dose of ACTH markedly influences the retention of AA in the adrenal gland without similarly altering retention of AA in other tissues. Furthermore, ACTH treatment causes both accelerated uptake and release of AA into the adrenals.

KEY WORDS: • vitamin C • adrenal ascorbic acid • ACTH • ascorbic acid

¹ This material is based upon work supported by the U.S. Department of Agriculture under Agreement No. 5901-0410-9-0326.

² Part of a thesis submitted by D. E. Kipp to the Graduate School in partial fulfillment for the requirements of the Ph.D. degree in the Division of Nutritional Sciences, Cornell University, Ithaca, NY.

³ A portion of this paper was presented at the 67th Annual Meeting of the Federation of American Societies for Experimental Biology [Kipp, D. E. & Rivers, J. M. (1983) The effect of adrenocorticotrophic hormone (ACTH) on ascorbic acid tissue distribution in the guinea pig. Fed. Proc. 42: 4527 (abs.)].

⁴ Present address and address for reprints: Department of Dietetics and Nutrition, University of Kansas Medical Center, 39th and Rainbow Blvd., Kansas City, KS 66103.

Manuscript received 10 February 1987. Revision accepted 1 May 1987.