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Metabolism of Methionine and Methionine Hydroxy Analogue by Porcine Kidney Fibroblasts^{1, 2, 3,}

Departments of Biochemistry and Animal Science, North Carolina State University, Raleigh, NC 27695

Porcine kidney fibroblasts in culture are able to utilize the 2-hydroxy analog of methionine (MHA) as the sole methionine source, but higher concentrations of MHA than methionine are required. The optimal concentration for growth with L-methionine ranges from 0.02 to 1.0 mM, whereas that for DL-MHA is 7.5 mM. At concentrations above 1.0 mM, L-methionine exhibits a toxic effect. Supplementing 1.0 mM L-methionine with DL-MHA allows greater growth than that provided by an equimolar concentration of L-methionine alone, and no toxic effect is seen at methionine source (DL-MHA) concentrations up to 7.5 mM. Early in the growth phase of cells where DL-MHA is the sole source of methionine, a 48-h lag period is observed over that seen in cells grown in the presence of 1.0 mM L-methionine. After the lag period, the MHA-supplemented cells double at the same rate as the methionine-supplemented cells. Studies with the purified isomers of D- and L-MHA indicate that only the D-isomer is utilized by these fibroblasts.

KEY WORDS: • L-methionine • D-methionine • methionine hydroxy analogue • pig • kidney fibroblasts

¹ Paper No. 10423 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7621. The use of trade names in this publication does not imply endorsement by the North Carolina Agriculture Research Service of the products named, or criticism of similar ones not mentioned.

² This research was funded partially by a research grant from Monsanto Industrial Chemicals, St. Louis, MO.

³ This report represents a part of the Ph.D. dissertation of C. L. Schreiner submitted to the Graduate School of North Carolina State University.

Manuscript received 18 February 1987. Revision accepted 15 May 1987.

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