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Journal of Nutrition Vol. 117 No. 6 June 1987, pp. 1098-1101
Copyright © 1987 by American Society for Nutrition
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Isoleucine Requirement of Young Growing Male Guinea Pigs1,2,

Linette Stelplugh Ayers3, John T. Typpo4 and Gary F. Krause*

Department of Human Nutrition, Foods and Food Systems Management * Department of Agronomy, University of Missouri, Columbia, MO 65211

The isoleucine requirement of young, growing male guinea pigs was investigated by use of crystalline amino acid diets containing 3.65% nitrogen. Three-week-old guinea pigs were fed one of eight crystalline amino acid diets ranging from 0.2 to 1.2% isoleucine for 22 d. Diets were evaluated on the basis of body weight changes, nitrogen retention, carcass weight, liver weight, hematocrit, hemoglobin and carcass and liver weights expressed as percentages of live body weight. A 0.5% level of dietary isoleucine (2.2% of total nitrogen x 6.25) was the lowest level fed that did not have a response significantly lower than the higher levels fed, and that generally promoted a thrifty and well-groomed appearance of the animals.

KEY WORDS: • isoleucine requirement • amino acids • guinea pigs

1 Contribution from the Missouri Agricultural Experiment Station, Journal Series No. 9899.

2 Presented at meetings of the Federation of American Societies for Experimental Biology in Atlantic City, New Jersey, in April 1978 [Stelplugh, L. A. & Typpo, J. T. (1978) Isoleucine requirement of the growing guinea pig. Fed. Proc. 37: 537 (abs. 1707)].

3 Present address: Lifewise, St. Luke's Hospital, 4230 Broadway, Kansas City, MO 64111.

4 Reprint requests should be sent to J. T. Typpo, Department of Human Nutrition, Foods and Food Systems Management, 16 Gwynn Hall, University of Missouri-Columbia, Columbia, MO 65211.

Manuscript received 23 July 1986. Revision accepted 29 January 1987.






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