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Influences of Dietary Restriction and Age on Liver Enzyme Activities and Lipid Peroxidation in Mice¹

Division of Toxicology and Physiology, University of California, Riverside, CA 92521 ^* Department of Pathology, University of California, Los Angeles, CA 90024

Dietary restriction extends maximum life span in rodents by unknown mechanisms. We compared livers from 12- and 24-mo-old mice fed control (C, 95 kcal/wk) or restricted (R, 55 kcal/wk) amounts of diet since 3 wk of age. We hypothesized that dietary restriction might alter the activity levels of enzymes with possible relevance to aging processes. The enzymes included several xenobiotic metabolizers, radical scavengers (catalase, superoxide dismutase, glutathione peroxidase), superoxide sources (xanthine oxidase, peroxisomal ß-oxidation of palmitoyl-CoA) and glucose-6-phosphatase. Lipid peroxidation (LP) was also measured. Comparing 12- and 24-mo-old mice, the strongest diet or age effect was an increased catalase activity for group R (42% higher at 12 mo, 64% at 24 mo). LP was clearly lower in group R at 12 mo (a 30% decrease) and somewhat lower (13%) at 24 mo than in group C. Similarly, in 12-mo-old C and R mice injected with either the P-450 inducer ß-naphthoflavone (ß-NF in corn oil) or with corn oil alone, R mice showed higher catalase activity (40–44%) and lower LP (43–46%) in both ß-NF-injected and vehicle-injected groups. These data suggest that if free radical damage is involved in aging, it may be a particular kind of damage, that is, that in part prevented by a selective increase in catalase activity.

KEY WORDS: • dietary restriction • age • catalase

¹ This study was supported by U.S. Public Health Service Research Grants AG-00424 and AG-04419 and by funds from the Life Extension Foundation.

Manuscript received 17 July 1986. Revision accepted 1 October 1986.

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