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Lipoprotein Lipase Activity in Skeletal Muscle and Brown Adipose Tissue of Pregnant and Lactating Rats1

Odette Champigny2 and Yvonne Hitier

Centre de Recherches sur la Nutrition du CNRS, 92190 Meudon-Bellevue, France

Changes in lipoprotein lipase (LPL) activity during pregnancy and lactation were followed in skeletal muscles and interscapular brown adipose tissue (BAT) of rats fed two diets differing in energy density (high carbohydrate or high fat). Rats were decapitated after 7, 19 or 21 d of pregnancy or after 3 or 12 d of lactation. Virgin rats and females separated from their litter just after delivery were used as nonpregnant and nonlactating controls, respectively. Blood was collected for determination of plasma glucose, triglycerides (TG) and nonesterified fatty acids (NEFA). Soleus, extensor digitorum longus (EDL), diaphragm and interscapular BAT were rapidly removed and frozen in liquid nitrogen for LPL activity measurement. LPL activity was not significantly higher in muscles and BAT of virgin rats fed the high fat diet than in those of rats fed the high carbohydrate diet. No significant change of skeletal muscle LPL activity was observed during pregnancy, regardless of the diet fed. Although BAT exhibited a transitory hypertrophy during pregnancy, its LPL activity was not significantly altered; during lactation BAT lost weight and its LPL activity dropped sharply when either diet was fed, leaving more TG available for milk production.

KEY WORDS: • pregnancy • lactation • high fat diet • lipoprotein lipase • skeletal muscles • brown adipose tissue

1 Supported in part by a grant from the Institute National de la Santé et de la Recherche Médicale (CRE 837010).

2 To whom correspondence should be sent.

Manuscript received 18 February 1986. Revision accepted 10 September 1986.






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