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Fatty Acid-Binding Protein Activity in Tissues from Pigs Fed Diets Containing 0 and 20% High Oleate Oil¹

Meats and Muscle Biology Section, Department of Animal Science, Texas A&M University, College Station, TX 77843

Eight pigs (four sets of littermates) were allotted to either a control sorghum-soybean meal diet or a test diet containing 20% canola oil. The pigs were slaughtered at approximately 100 kg live weight. Subcutaneous adipose tissue, longissimus dorsi muscle and liver samples were collected immediately after slaughter. Fatty acid-binding protein (FABP) was measured in 104,000 x g supernatant fractions. Characterization of the FABP assay with liver supernatants indicated that FABP exhibited sigmoidal kinetics, with an apparent K_d of 0.75 µM. Doubling the protein amount in the assay did not affect the FABP activity. FABP was demonstrated in liver, with an activity of 3448 pmol bound/g liver, and in adipose tissue, with an activity of 276 pmol bound/g adipose tissue. No activity was observed in the muscle tissue. FABP activity of liver and adipose tissue from swine fed 20% canola oil was higher on a per gram tissue basis than that in tissues from pigs fed the control diet.

KEY WORDS: • fatty acid-binding protein • swine • monounsaturated fatty acids

¹ Technical article No. 22427, Texas Agricultural Experiment Station.

² Present address: Animal Science Department, Texas A&M University, College Station, TX 77843.

³ Present address: R. L. Hruska U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE 68933.

Manuscript received 9 February 1987. Revision accepted 10 August 1987.