QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stein, T. P. Articles by Melnick, G. Search for Related Content PubMed PubMed Citation Articles by Stein, T. P. Articles by Melnick, G.

Metabolism of Nonessential ¹⁵N-Labeled Amino Acids and the Measurement of Human Whole-Body Protein Synthesis Rates¹

T. P. Stein^*,,, R. G. Settle, J. A. Albina, D. T. Dempsey and G. Melnick

^* Department of Surgery, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Camden, NJ 08103 Department of Surgery Department of Otorhinolaryngology, University of Pennsylvania, Philadelphia, PA 19104

Eight ¹⁵N-labeled nonessential amino acids plus ¹⁵NH₄Cl were administered over a 10-h period to four healthy adult males using a primed-constant dosage regimen. The amount of ¹⁵N excreted in the urine and the urinary ammonia, hippuric acid and plasma alanine ¹⁵N enrichments were measured. There was a high degree of consistency across subjects in the ordering of the nine compounds based on the fraction of ¹⁵N excreted (Kendall coefficient of concordance W=0.83, P < 0.01). Protein synthesis rates were calculated from the urinary ammonia plateau enrichment and the cumulative excretion of ¹⁵N. Glycine was one of the few amino acids that gave similar values by both methods.

KEY WORDS: • ¹⁵N • nonessential amino acids • protein synthesis

¹ Supported by USPHS grant #AM33431 and NASA contract #9-16874.

Manuscript received 21 June 1985. Revision accepted 7 May 1986.

This article has been cited by other articles:

				Y. Lamers, J. Williamson, L. R. Gilbert, P. W. Stacpoole, and J. F. Gregory III Glycine Turnover and Decarboxylation Rate Quantified in Healthy Men and Women Using Primed, Constant Infusions of [1,2-13C2]Glycine and [2H3]Leucine J. Nutr., December 1, 2007; 137(12): 2647 - 2652. [Abstract] [Full Text] [PDF]