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Effect of Zinc Status on the Binding of Prostaglandins to Ovarian Membranes and Intact Platelets of Pregnant Rats^1,2,

Department of Biochemistry, University of Missouri, 322-A Chemistry Building, Columbia, MO 65211

The effects of zinc deficiency on the binding of PGF₂ to ovarian membranes and of PGE₁ to intact platelets prepared from pregnant rats were examined. On d 21 of gestation, rats fed low zinc (<1 ppm) diets throughout pregnancy had prostaglandin receptor characteristics significantly different from those of rats fed control diets (100 ppm zinc). The number of binding sites for PGF₂ was higher in the ovarian membranes prepared from deficient rats than in those from controls, whereas the affinity was not affected. In washed platelets from zinc-deficient rats there were fewer PGE₁ binding sites but greater affinity for PGE₁ than in platelets from controls. These results demonstrate that zinc status affects prostaglandin binding to membranes. The effects are not uniform with regard to different tissues and prostaglandin receptors but suggest an effect of zinc status on plasma membranes.

KEY WORDS: • zinc deficiency • pregnancy • prostaglandin F₂ • prostaglandin E₁ • receptor binding • rat

¹ This work was supported in part by the Missouri Agricultural Experiment Station, Journal Series No. 9921 and by the Department of Health and Human Services, Grant No. HL 11614.

² Presented in part at the 1985 Federation of the American Societies for Experimental Biology meeting in Anaheim, CA; Fed. Proc. 44, 542 (abs. 768).

³ Present address: Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, 351 FitzGerald Building, Toronto, Ontario, M5S 1A8, Canada.

⁴ To whom correspondence should be directed.

Manuscript received 12 July 1985. Revision accepted 19 March 1986.