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Dietary Modulation of Epidermal Growth Factor Action in Cultured Pancreatic Acinar Cells of the Rat^1,2,3,

Departments of Nutrition and Food Science ^* Internal Medicine, University of Arizona, Tucson, AZ 85721

The effects of epidermal growth factor (EGF) and its interaction with dietary adaptation were examined in pancreatic acinar cells isolated from rats fed for 1 wk diets with 67% kcal as fat (HF), 67% kcal as carbohydrate (HC), or unrefined, closed formula diet (UD). Cells were cultured for 48 h in serum-free medium containing 4.2 nM (control), 42 pM, 0.42 nM or 42 nM EGF. EGF at the concentrations tested did not affect cellular protein, DNA, amylase and lipase. In freshly isolated and cultured cells, specific activity of intracellular phenylalanine was affected by diet with the highest specific activity in HF cells, but was not affected by EGF. In freshly isolated cells, EGF increased the rate of phenylalanine incorporation into cellular protein only in HF cells at 42 nM. In cultured cells, EGF biphasically increased phenylalanine incorporation at 42 pM and 42 nM in UD and HC cells, but only increased phenylalanine incorporation in HF cells at 42 nM. These data suggest that diet can alter the response of pancreatic acinar cells to EGF. Dietary alterations of cellular responsiveness to regulatory peptides may participate in pancreatic regulation and dietary adaptation in vivo.

KEY WORDS: • exocrine pancreas • acinar cell • epidermal growth factor • dietary adaptation

¹ This research supported in part by Hatch Project 174076 of the Arizona Agriculture Experiment Station to P. M. Brannon, National Institutes of Health Research Grants AM32690 to P. M. Brannon and AM32561 to M. Korc from the National Institute of Arthritis, Diabetes, Digestive and Kidney Disease, American Heart Assn., Arizona Affl. grant-in-aid and Biomedical Research Support Grant to P. M. Brannon.

² Presented in part at the 68th Annual Meeting of the Federation of American Societies for Experimental Biology (FASEB), St. Louis, MO, April 1984 [Demarest, A. S., Brannon, P. M. & Korc, M. (1984) Effects of epidermal growth factor on pancreatic acinar cells in serum-free culture. Fed. Proc. 43, 1073 (abs.)] and at the 69th Annual Meeting of FASEB, Anaheim, CA, April 1985 [Demarest, A. S., Sabb, J. E. & Brannon, P. M. (1985) Effect of diet on cellular responsiveness to epidermal growth factor in pancreatic acinar cells. Fed. Proc. 44, 931 (abs.)].

³ Part of a thesis submitted to the Graduate College of the University of Arizona in partial fulfillment of the requirements for the M.S. degree.

Manuscript received 22 August 1985. Revision accepted 10 February 1986.