QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Dills, W. L. Articles by Kingsley-Hickman, P. B. Search for Related Content PubMed PubMed Citation Articles by Dills, W. L., Jr. Articles by Kingsley-Hickman, P. B.

Dietary Potentiation of the Antifertility Effects of 5-Thio-D-glucose in Male Rats¹

William L. Dills, Jr.^{2,*, ,}, William E. Berndtson, Thomas R. Covey^3, and Peter B. Kingsley-Hickman^4,

^* Department of Chemistry, Southeastern Massachusetts University, North Dartmouth, MA 02747 Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853 Department of Animal and Nutritional Sciences, University of New Hampshire, Durham, NH 03824

Male rats of proven fertility were fed the following diets for 28 d either with or without 0.075% 5-thioglucose (5-THG): AIN-76 diet (A76): a diet with 13% casein, 2% glucose and the balance of the calories as free corn oil fatty acids from corn oil (2G); and a similar diet, isocaloric with 2G, with the glucose level increased to 20% (20G). The diets alone without 5-THG had no influence on any of the parameters measured. Body weight gain was lower in rats fed diets containing 5-THG than in those fed diets without 5-THG. In rats fed A76, the only 5-THG effects on male reproductive tract (MRT) tissues was the appearance of testicular multinucleate giant cells (MGC). In rats fed either 2G or 20G, the MRT effects of 5-THG included the appearance of MGC, a lower number of germ cells at most stages of maturation, lower sperm counts and biochemical changes in testis slices and in germ cell preparations compared to rats not fed 5-THG. There were fewer Step 7 spermatids in rats fed 5-THG in 2 G than in those fed 5-THG in 20G. It is concluded that the MRT toxicity of 5-THG is influenced by diet, being potentiated by the low protein diet high in free fatty acids and, to a lesser extent, by low glucose levels within these diets.

KEY WORDS: • antifertility • 5-thioglucose • carbohydrate-deficient diets

¹ Supported by NIH grants HD-10967, AM-20882, and CA-26755, NIH training grant T32-AM-07158 to the Division of Nutritional Sciences, Cornell University, a grant from the Lester G. Wood Foundation, grant 14–83 from the SMU Foundation and funds from the Research Committee at SMU. A portion of this work was presented at the 68th Annual Meeting of the Federation of American Societies for Experimental Biology, St. Louis, MO, April 1–6, 1984. Dills, W. L., Berndtson, W. E., Covey, T. R. and Kingsley-Hickman, P. B. (1984) Dietary potentiation of the antifertility effects of 5-thioglucose in rats. Fed. Proc. 43, 688 (abs. 2357).

² Reprint requests: Dr. William L. Dills, Jr., Department of Chemistry, Southeastern Massachusetts University, North Dartmouth, MA 02747.

³ Current address: 925 Warren Rd., Equine Drug Testing, Cornell University, College of Veterinary Medicine, Ithaca, NY 14850.

⁴ Current address: Gray Freshwater Biological Institute, County Roads 15 and 19, Navarre, MN 55392.

Manuscript received 25 January 1985. Revision accepted 23 December 1985.