QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Baly, D. L. Articles by Hurley, L. S. Search for Related Content PubMed PubMed Citation Articles by Baly, D. L. Articles by Hurley, L. S.

Pyruvate Carboxylase and Phosphoenolpyruvate Carboxykinase Activity in Developing Rats: Effect of Manganese Deficiency^1,2,

Department of Nutrition, University of California, Davis, CA 95616

The activities of two liver gluconeogenic enzymes, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) as well as plasma glucose were measured in manganese-sufficient and manganese-deficient rats from birth to 30 d of age. Initial (d 0) PC activity was similar in the two groups. PC activity increased 1.5-fold in control pups and 2.4-fold in manganese-deficient pups from d 0 to 3 postnatally. PEPCK activity increased 1.5-fold in control pups and 2.2-fold in manganese-deficient pups from d 0 to 3. By d 8, the activity of PEPCK in manganese-deficient pups was 60% of control levels. Compared to control pups, plasma glucose concentration was lower in manganese-deficient pups on d 1 and 2, coinciding with a period of high neonatal mortality. These findings suggest that glucose homeostasis in the newborn may be compromised by manganese deficiency and may support the concept that changes in cellular manganese concentration may be important in the regulation of carbohydrate metabolism.

KEY WORDS: • manganese • gluconeogenesis • glucose • pyruvate carboxylase • phosphoenolpyruvate carboxykinase • development

¹ Supported in part by National Institutes of Health Grant HD-02355 from the National Institute of Child Health and Human Development.

² Presented in part at the 74th annual meeting of the American Society for Biological Chemists in San Francisco, CA, in June 1983.

³ To whom reprint requests should be sent.

Manuscript received 12 July 1984. Revision accepted 11 March 1985.