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Cholesterol Transport in Mesenteric Lymph of the Preruminant Calf: Contribution of Cholesterol Transposition from Blood to Lymph^1,2,3,

Nutritional Physiology Group, Animal Science Department, Iowa State University, Ames, IA 50011

Eight preruminant male calves were prepared surgically with lymphatico-venous shunts and re-entrant gallbladder to proximal duodenum shunts. Liquid diets were formulated to contain 12.5% dried skim milk (SM) or 10.5% SM to which was added 2% soybean oil (SBO), milk fat (MF) or beef tallow (T). Two calves were assigned to each dietary treatment. Transposition of cholesterol from blood capillaries to intestinal lymph was determined by injection of 100 µCi [4-¹⁴C]-cholesterol into the blood of calves at feeding time. To avoid recirculation of [4-¹⁴C]-cholesterol via the enterohepatic circulation, bile was diverted and replaced with bile from a donor calf fed an identical diet. For the SM, SBO, MF and T diets, respectively, cholesterol transposed from capillaries was 44, 61, 36 and 48% of the cholesterol transported in the mesenteric lymph. When cholesterol synthesis in response to test diets was calculated, we found that intestinal cholesterol synthesis is less when calves are fed SM or SBO than when fed T or MF.

KEY WORDS: • calf • lymph • bile • cholesterogenesis • cholesterol transposition

¹ Journal paper No. J-11367 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, Project No. 2505.

² This research was supported by the Science and Education Administration of the U.S. Department of Agriculture under Grant No. 2338 from the Competitive Research Grants Office.

³ A portion of these data was presented at the Annual Meeting of the American Dairy Science Association, 1982, University Park, PA. J. Dairy Sci. 65 (Suppl. 1), 196 (abs. P273).

⁴ Author to whom reprint requests should be addressed.

Manuscript received 2 May 1984.