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Journal of Nutrition Vol. 115 No. 11 November 1985, pp. 1535-1539
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Fatty Acid-Binding Protein Activities in Bovine Muscle, Liver and Adipose Tissue1,2,

Stephen B. Smith, Peg A. Ekeren and James O. Sanders

Meats and Muscle Biology Section, Department of Animal Science, Texas A&M University, College Station, TX 77843

Subcutaneous adipose tissue, sternomandibularis muscle and liver were obtained from steers immediately postmortem. Muscle strips and adipose tissue snips were incubated with 0.75 mM [1-14C]palmitate and 5 mM glucose. Muscle strips esterified palmitate at the rate of 2.5 nmol/min per gram tissue, which was 30% of the rate observed for adipose tissue. Fatty acid-binding protein activity was measured in 104,000 x g supernatant fractions of liver, muscle and adipose tissue homogenates. Muscle and adipose tissue fractions bound 840 and 140 pmol [1-14C]palmitoyl-CoA per gram tissue, respectively. Fatty acid-binding protein activity was greater in adipose tissue than in muscle when data were expressed per milligram protein (35 vs. 13 pmol palmitoyl-CoA bound per milligram of soluble protein, respectively). Fatty acid binding-protein activity was correlated with the rate of palmitate esterification within each tissue. Liver contained the highest fatty acid-binding protein activity (13,000 pmol palmitoyl-CoA bound per gram tissue and 215 pmol palmitoyl-CoA bound per milligrams soluble protein).

KEY WORDS: • fatty acid-binding protein • bovine • adipose tissue • muscle • liver

1 Technical article no. 20247, Texas Agricultural Experiment Station.

2 This research was supported in part by National Institutes of Health Biomedical Research Support Grant 55360 to Texas A&M University.

Manuscript received 4 April 1985. Revision accepted 1 August 1985.






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