QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bruno, J. V. Articles by Farrell, P. M. Search for Related Content PubMed PubMed Citation Articles by Bruno, J. V. Articles by Farrell, P. M.

Lung Surfactant Phospholipids as Related to Hydration and Choline Status of Fasted Rats¹

James V. Bruno^*, Kathleen E. McMahon^{*, ,} and Philip M. Farrell^{*, ,}

^* Department of Pediatrics Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53792

Hydration status and choline nutrition were evaluated relative to the concentration of lung surfactant phosphatidylcholine (PC) in fasted rats. Rats deprived of food for 72 h showed lower voluntary water consumption and consistently lower levels of both PC and total phospholipid (TPL) present in isolated pulmonary surfactant than ad libitum-fed controls, although the ratio of surfactant PC to TPL and the residual PC and TPL concentrations were not different. Higher hematocrit values observed in the fasted animals were not altered by the administration of water or saline by orogastric tube nor was the low surfactant PC level corrected by fluid therapy. Evidence of choline deficiency was demonstrated in the fasted rats as there was a significant shift in hepatic PC:phosphatidylethanolamine; however, plasma choline levels did not change. The administration to fasted animals of up to 2.4 mmol of choline chloride via the drinking water (containing 60 mM choline) or an orogastric tube did not affect the plasma choline concentrations or the production of lung surfactant PC.

KEY WORDS: • lung • surfactant • choline • starvation • dehydration

¹ This work was supported in part by a medical student traineeship for JVB from the National Institutes of Health (AM 07389-01) awarded to Professor Harold Deutsch, Department of Physiological Chemistry, University of Wisconsin. PMF was supported by Pulmonary SCOR grant P50-HL-27348 from the National Institutes of Health and KEM by a Foremost-McKesson Fellowship awarded by the American Institute of Nutrition.

Manuscript received 3 July 1984.