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Distribution of ¹⁴C after Oral Administration of [1-¹⁴C]Linoleic Acid in Rats Fed Different Levels of Essential Fatty Acids

Nutrition Laboratory, National Food Administration, P.O. Box 622, S-751 26 Uppsala, Sweden

Rats from an inbred Sprague-Dawley strain were fed semisynthetic diets with a low [0.3 energy percent (en %)], normal (3 en %) or high (10 en %) content of essential fatty acids (EFA) for at least three generations. Twenty-nine- to 33-day-old male rats were given a single intragastric dose of [1-¹⁴C]linoleic acid in olive oil, and the respiratory CO₂, urine and feces were collected for 46 hours (expt 1) or 20 hours (expt 2). The ¹⁴C activity in respiratory CO₂, feces, urine and the carcass was determined in both experiments. In experiment 2 it was also measured in samples of the brown fat, liver, adrenals, white fat, skeletal muscles and brain. In both experiments the rats fed the low EFA diet retained significantly more ¹⁴C activity than the rats fed the normal or high EFA diets. In all groups the concentration of label was highest in the brown fat and the adrenals, but the above differences among the groups with respect to ¹⁴C retention were mainly observed in the liver, skeletal muscles and brain.

KEY WORDS: • essential fatty acid deficiency • linoleic acid • oxidation • tissue incorporation • rat

Manuscript received 20 March 1984.