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Involvement of D-Amino-Acid Oxidase in D-Amino Acid Utilization in the Mouse

Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi 321-02, Japan

Mutant ddY/DAO^- mice lacking D-amino-acid oxidase were examined for their ability to utilize D-phenylalanine in place of its L-isomer. Chemically defined milk powder devoid of phenylalanine was used as a basal diet. Adult ddY/DAO^- mice and normal ddY/DAO⁺ mice having D-amino-acid oxidase lost weight every day when fed this diet. However, they maintained their weight when fed the milk powder diet supplemented with 0.33% L-phenylalanine. Although adult ddY/DAO⁺ mice maintained their weight when fed the milk powder diet supplemented with 0.33% D-phenylalanine, ddY/DAO^- mice lost weight while feeding on this diet, which indicated that the ddY/DAO^- mice could not utilize D-phenylalanine in place of its L-isomer. Both ddY/DAO^- and ddY/DAO⁺ mice maintained their weight when feeding on the milk powder diet enriched with 0.41% sodium phenylpyruvate, which suggested to us that the ddY/DAO^- mice had the ability to invert this -keto acid to L-phenylalanine. Therefore, these results indicate that D-amino-acid oxidase is indispensable for D-amino acid utilization. This is consistent with the concept that D-amino acids are oxidatively deaminated to the corresponding -keto acids by D-amino-acid oxidase and that these keto intermediates are then asymmetrically reaminated to their L-amino acids.

KEY WORDS: • D-amino-acid oxidase • D-phenylalanine • weight change • mutant mouse

Manuscript received 21 February 1984.

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