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Metabolism of [⁷⁵Se]Selenite by Rhesus Monkeys^1,2,

Department of Agricultural Chemistry, Oregon State University, Corvallis, OR 97331

The metabolism of [⁷⁵Se]selenite was studied in rhesus monkeys. In blood samples taken various times after injection, Sephadex G-150 gel filtration revealed that the majority of the ⁷⁵Se was associated with hemoglobin and low-molecular-weight compounds in erythrocytes up to 24 hours after selenium injection. Subsequently a gradual increase of ⁷⁵Se occurred in glutathione peroxidase (GSH-Px) with a concurrent decrease of label with hemoglobin. In contrast to the erythrocytes, over 80% of the labeled selenium in plasma was associated with one peak 3 hours and later after injection. This major protein eluted at a position similar to GSH-Px on gel filtration, but subsequent chromatography on diethylaminoethyl (DEAE)-Sephacel separated the radiolabeled protein from GSH-Px. Gel filtration of heart, muscle, brain and pancreas cytosol revealed two major selenium-containing proteins, whereas one was predominant in liver and kidney. The major selenium peak was associated with GSH-Px in liver but not in the kidney. GSH-Px activity with either organic or inorganic peroxides as substrates and glutathione transferase activity were higher in liver than kidney.

KEY WORDS: • [⁷⁵Se]selenite metabolism • monkeys • erythrocytes • plasma • tissues • glutathione peroxidase

¹ Published with the approval of the Oregon State Agricultural Experiment Station as Technical Paper No. 6964.

² Supported by U.S. Department of Agriculture Grant No. 8000566 from the Competitive Research Grants Program and, Public Health Service Research Grant Number AM 21037 from The National Institute of Arthritis, Metabolism and Digestive Diseases, and by Public Health Service Research Grant number NS 07413 from the National Institute of Neurological and Communicative Disorders and Stroke.

Manuscript received 15 March 1984.