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Long-Term Effects of Inadequate and Excessive Dietary Ascorbate on Bile Acid Metabolism in the Guinea Pig^1,2,

Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853

The effects of long-term chronic ascorbic acid deficiency and excessive ascorbic acid consumption on bile acid metabolism and biliary lipid composition were studied in guinea pigs. Male, weanling guinea pigs were fed a cereal-based scorbutigenic diet for 19 or 21 weeks. Ascorbic acid was administered either orally at 0.15 (group A) or 2.0 (group B) mg/100 g body weight, or it was mixed in the diet at levels of 500 (group C), 16–22 (group D), or 20,000 mg/kg (group E). Chronic ascorbic acid deficiency (groups A and D) caused depression of hepatic cytochrome P-450 levels and elevation of plasma cholesterol. Excessive ascorbate consumption did not alter these parameters relative to control levels. In contrast to results obtained in guinea pigs fed low or high amounts of ascorbate for 7–9 weeks, prolonged consumption of inadequate or excessive ascorbate resulted in little or no change in bile acid metabolism and biliary lipid composition except that bile acid pool size was increased 12% as a result of excessive ascorbate ingestion. Results of the present study suggest that there may be important differences in the guinea pig's metabolic response to ascorbic acid deficiency and ascorbic acid excess, depending on the length of the experimental period.

KEY WORDS: • ascorbic acid • bile acid • cholesterol

¹ Supported in part by National Institutes of Health Predoctoral Traineeship No. GM-01221 (David E. Holloway).

² From a thesis submitted by David E. Holloway in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Cornell University, 1980.

Manuscript received 24 March 1984.