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The Effects of High Dietary Zinc and Copper Deficiency on the Activity of Copper-Requiring Metalloenzymes in the Growing Rat^1,2,

Mary R. L'Abbé^3,*,, and Peter W. F. Fischer^*

^* Nutrition Research Division, Bureau of Nutritional Sciences, Food Directorate, Health and Welfare Canada, Ottawa, Ontario, Canada K1A 0L2 Department of Animal Science, MacDonald Campus of McGill University, Ste. Anne de Bellevue, Quebec, Canada H9X 1C0

The effects of feeding rats high levels of dietary zinc (240 mg Zn/kg diet) on the activities of the copper-requiring metalloenzymes: ceruloplasmin (Cp), cupro-zinc superoxide dismutase (SOD) and cytochrome c oxidase (CCO) were determined. These were compared with those seen during copper deficiency (induced by feeding 0.6 mg Cu/kg diet). The activities of Cp in serum, Cu-Zn SOD in liver and heart, and CCO in heart were significantly reduced in both the high zinc and copper-deficient groups by 2 weeks, when compared to the activities seen in rats fed the control diet (6 mg Cu, 30 mg Zn/kg diet). In animals fed the high zinc diet, the reduction in heart CCO activity followed the decrease seen in heart copper concentration, whereas in blood and liver, the reductions in Cp and SOD activities, respectively, were greater than the reductions seen in copper concentration. Thus, the results of this experiment demonstrate that with high dietary zinc, the reductions seen in the activity of copper-requiring metalloenzymes were similar to those seen during copper deficiency.

KEY WORDS: • copper • deficiency • zinc • ceruloplasmin • superoxide dismutase • cytochrome c oxidase

¹ Publication No. 171 of the Bureau of Nutritional Sciences.

² A portion of these results was previously presented at the 74th Annual Meeting of the American Society of Biological Chemists, San Francisco, June 5–9, 1983. Fischer, P. W. F. and L'Abbé, M. R. (1983) Fed. Proc. 42, 2270 (abs. 2994).

³ This work was carried out in partial fulfillment of the requirements for the M.Sc. degree from McGill University.

Manuscript received 20 September 1983.

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