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Absorption and Metabolism of Adenine, Adenosine-5'-Monophosphate, Adenosine and Hypoxanthine by the Isolated Vascularly Perfused Rat Small Intestine^1,2,

Department of Food Science and Nutrition, and Department of Biochemistry, University of Minnesota, St. Paul, MN 55108

Intestinal vascular perfusion and in vivo live animal experiments were conducted in order to evaluate the nature and extent of the intestinal metabolism of adenine, adenosine, adenosine-5'-monophosphate (AMP) and hypoxanthine in the rat. Radiolabeled purine substrates were administered intralumenally. Intestinal contents, tissue and/or portal flow were collected and evaluated for resultant purine metabolites by liquid and paper chromatography and paper electrophoresis. Adenosine, AMP and hypoxanthine were quantitatively metabolized to end products (primarily uric acid) within 15 minutes after administration. In contrast, the metabolism of adenine to uric acid was considerably slower. Up to 20% of the administered adenine was recovered unmetabolized in the portal vasculature. Nonetheless uric acid was the primary metabolite recovered from the portal circulation in the isolated intestine regardless of the purine substrate or concentration administered. Since lumenal inosine concentrations rose sharply with increasing doses of AMP, either transport or metabolism of inosine is a rate-limiting step in the intestinal metabolism of purines to uric acid in the rat. Finally, the large percentage of the radiolabel in uric acid recovered in the lumen is consistent with the hypothesis that the intestine is an extrarenal site for purine excretion.

KEY WORDS: • adenine • adenosine • AMP • hypoxanthine • intestine • uric acid

¹ University of Minnesota Agricultural Experiment Station Journal Article No. 13,603.

² Supported in part by research funds of the Graduate School of the University of Minnesota.

³ To whom reprint requests should be sent: Dept. Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St. Paul, MN 55108.

Manuscript received 28 September 1983.

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