QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gregory, J. F. Articles by Day, B. P. F. Search for Related Content PubMed PubMed Citation Articles by Gregory, J. F., III Articles by Day, B. P. F.

Fluorometric Determination of Folacin in Biological Materials Using High Performance Liquid Chromatography^1,2,

Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611

A procedure was developed for the quantitation of the major folacin compounds present in foods and other biological materials. Extraction conditions were selected to provide conversion of 10-formyltetrahydrofolic acid (10-HCO-H₄folic acid) to 5-HCO-H₄folic acid. Polyglutamyl folates were deconjugated with hog kidney conjugase. The resulting folacin monoglutamates were separated by reverse-phase high performance liquid chromatography after extract purification on an anion exchange column. Detection of H₄folic acid and its substituted derivatives was performed by monitoring the native fluorescence of the reduced folates in the acidic mobile phase. Folic acid, H₂folic acid and also H₄folic acid were measured fluorometrically by using an oxidative postcolumn derivatization system in series with the first fluorometer. Detection limits ranged from 0.03 to 2.3 pmol/100 µl injection for the various folates. The validity of the method was supported by recovery and fluorescence spectral studies and by comparison with Lactobacillus casei assays for a variety of samples. Analysis of rat liver following a single pulse dose of ³H-labeled folic acid indicated large differences between the patterns of radiolabeled and endogenous folates.

KEY WORDS: • folacin • high performance liquid chromatography • folate distribution • food analysis

¹ Supported in part by Grant No. 59-2121-1-1-766-0 from the U.S. Department of Agriculture. Science and Education Administration, Competitive Research Grants Office and funds from the Florids Agricultural Experiment Station.

² Florida Agricultural Experiment Station Journal Series No. 5280.

Manuscript received 26 July 1983.

This article has been cited by other articles:

				E. P. Quinlivan, S. Roje, G. Basset, Y. Shachar-Hill, J. F. Gregory III, and A. D. Hanson The Folate Precursor p-Aminobenzoate Is Reversibly Converted to Its Glucose Ester in the Plant Cytosol J. Biol. Chem., May 30, 2003; 278(23): 20731 - 20737. [Abstract] [Full Text] [PDF]

				G. Basset, E. P. Quinlivan, M. J. Ziemak, R. Diaz de la Garza, M. Fischer, S. Schiffmann, A. Bacher, J. F. Gregory III, and A. D. Hanson Folate synthesis in plants: The first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I PNAS, September 17, 2002; 99(19): 12489 - 12494. [Abstract] [Full Text] [PDF]